Sample Tests
We check the service from start to finish independently from the laboratory. This gives a feel
for turn round, shows the style of report you will receive and an understanding of the types of
results seen in the UK population including impact of supplements.
February 2024
Secret shopper Jon
Comments
Jon is
a 67 year old white male.
•
In
January his level was ‘Insufficient’ and
this was with a daily dose of 2 x 25 µg
D3.
•
Jon increased his dose to 3 x 25 µg
daily for 4 weeks and then repeated the
test. This higher dose saw a level of 55
nmol/L.
•
On 28th February he reduced the
dose down to 1 x 25 µg/day. We will
repeat the test in a month or so to see
how this impacts levels.
January 2024 - Turn Round and Levels
Secret shopper Jon
Comments:
•
Jon has sent in 6 samples over the last few
years. In January 2024 he had been taking
2 x 25 µg Boots Vitamin D High Strength
tablets ‘most mornings’.
•
It is surprising to find levels are not even
up to the basic 50 nmol/L. Jon will try and
remember to take 3 x tablets now every
morning for 4 weeks and repeat the test in
middle of February.
May 2021
Secret Shopper: Jon
A 64 year old who we reported on in February when his level was 70 nmol/L. Of that 40 nmol/L was due to D2
supplements.
Comment:
Turn round is a little longer than the laboratory target and the email with the results apologised for this, and explains that
they are experiencing unprecedented demand due to additional Covid-19 related work. Hopefully turn round will come
back down over the next week or so.
Jonathan has not taken any Vitamin D2 supplements since 17th February and 61 days later the residual level of D2 =
14.9 nmol/L shows a long half life of a little over 30 days. Vitamin D is fat soluble and stays in the body stored in your fat a
lot longer than water soluble vitamins. Remember that Vitamin D2 is usually undetectable unless you take it as a
supplement. The 2.8 nmol/L on all the other Secret Shopper results is the limit of detection.
March 2021
Secret Shopper: Jen
A 31 year old specialist doctor in anaesthetics in south London. We tested her in December and she had a deficient result
of just 23.4 nmol/L. Jen started a 4000 iu/day vitamin D tablet. We retested three months later when her vitamin D
supplements should have have come to the ‘steady state’.
Comment:
Jen’s total vitamin D level is great now. Three months on 4000 iu of D3 supplements has brought a deficient level up to
one that is reasonable. Jen herself has also noticed the troublesome acne she has suffered for years is receding. She
‘Googled it’ and points to yet another association with Vitamin D - a number of studies have reported this including a
paper from 2016 here…
It is likely that Jen has a genetic cause for a low vitamin D. Her father, Jonathan below, also has a very low level without
supplements and one of Jen’s siblings - Rachael below, also has low levels without supplements.
Turn Round:
The sample took over a week to get from South London to the Birmingham laboratory. Unfortunately first class post from
some parts of the country is very ‘lumpy’ at present - to borrow a phrase from Covid-19 press conferences!
Secret Shopper Laura
A 35 year old town planner living in the West Midlands. Takes 25 ug (1,000 IU) Boots ‘High Strength Vitamin D’ every day
all though the year. Tested as her two sisters (Rachael and Jen) below and her father (Jonathan) all have naturally low
vitamin D level.
Comment: Considering the daily dose of supplements this is not a very high level. It is likely that
Laura would be in the ‘Insufficiency’ category without supplements. We will retest her sister Jen
later in March to see if supplements have sorted out her very low level from January.
Turn Round: The laboratory has had machine issues and with lock-down servicing can take longer
at present. The turn round at 14 working days was outside the laboratory target but the lab is
working hard to get back on course. In the longer term an additional LC-MS/MS and extra staff are
going to help keep up with continuing demand both for public testing and R&D studies.
Secret shopper: Jonathan
A 64 year old Clinical Scientist recently retired from the NHS and now working on T cell Covid-19
reactivity. The father of Jen and Rach below who both had low levels. We await the return of his
third daughter’s result - she is 7 months pregnant so lets hope it comes back at a good level!
Jonathan is known to run a low vitamin D unless he takes supplements. For Secret Shopper he
kindly switched to D2 supplements in January, taking 6000 IU/day for 5 weeks before this test. The
sample was taken two days after supplements were stopped. He had previously been taking 2000
IU/day of a D3 supplement.
Comment:
This is the first Secret Shopper with a measurable Vitamin-D2. All the others have been reported by
the lab as 2.8 nmol/L which is simply the limit of detection of the method. While his total level is
reasonable at 70 nmol/L the D3 alone would have given a low result. With two of his daughters
having a low vitamin D as well one must consider a genetic link to this family tendency to low
vitamin D. We will retest at the end of April to see if the D2 has all cleared and what his natural
level of Vitamin D is.
Turn round
The lab time was 9 working days which is at the longer end of the turn round target. In February
You Tube presenter Dr John Campbell used the test on his channel causing a surge in interest in the
service from the general public. Things should be back to normal over the next few weeks.
February 2021
Secret shopper: Rach
A 37 year old hospital consultant living on the south coast with an outdoor lifestyle. The eldest
sister of our DEFICIENT trainee anaesthetist Jen (see below - January secret shopper No. 1)
Rach takes an oral supplement “when she remembers”.
Comment
Rach has a low level of Vitamin D just getting to the ‘INSUFFICIENT’ level. Not as low as her
younger sister below ,who had a level of just 23.4 nmol/L. Not great when you consider she does
take some D3 oral tablets sporadically, and also spends weekends doing lots of things outside.
This is an interesting finding as these two sisters have a father who has been shown to have very
low vitamin D levels over many years. He has changed to D2 oral supplements for a couple of
months to see what his natural D3 level is these days. Remember Vitamin D3 is made by your skin
as well as being in food/supplements. Vitamin D2 is only obtained from vegetable products and
most people have an undetectable level. Rach and Jen have another sister - currently six months
pregnant, and she will be our second secret shopper for February in a few days time.
Turn Round
The in-lab time of 5 working days is better than the ‘up to 10 working days’ lab target. However the
laboratory saw huge demand for test packs towards the end of February which may impact on turn-
round in early March. If you are missing a result do check your spam folders, as the automated
system sending the emails is sometimes considered as spam by local systems.
January 2021 No.3
Secret shopper: Karen
A 61 year old Solihull resident who has kept strictly to lock-down rules over the last 10
months. Karen is aware of the interest in Vitamin D and possible severity of Covid-19
infection. She takes four oral supplements daily, two of which contain Vitamin D: Boots ‘High
Strength Vitamin D3’ and Aldi ‘ Heart Health, Omega 3 fish oil’.
Comment
Karen has achieved the highest level of Vitamin D of all our secret shoppers to date. It is a
level often seen in the laboratory and and such levels are associated with Vitamin D3
supplements being taken. The Boots tablet has 25 ug Vitamin D3 but the amount in the fish
oil is not stated - but looks like it does the job!
•
Do remember there are two oral supplements on the market. D3 comes from animal
sources - often extracted from sheep’s wool. D2 is extracted from vegetable sources.
•
The body also produces D3 in your skin due to UV light. Unless you take a Vitamin D2
supplement your D2 level is usually low. The 2.8 nmol/L you see in all the reports so far
is actually the ‘detection limit’ of the method.
•
We have an upcoming secret shopper who has switched from D3 to D2 supplements
to demonstrate this. Please do not ‘do this at home’ though as D3 is thought to be
better to supplement, unless advised otherwise by a health care professional.
Turn round
Karen posted her sample on a Sunday night in the reply paid first class post pack supplied. It
arrived in the laboratory fast. Analysis was prompt with a turn round of 5 working days. The
laboratory is to be complimented - the staff are working in very different ways with social
distancing and face masks mandatory, and the workload is very high with NHS and research
samples and our service to the public.
January 2021 No. 2
Secret Shopper: Audrey
An 89 year old great grandmother who after a stroke a year ago has been a care-home
resident in the South-West. She takes a daily Vitamin D supplement, and has done so for
many years. She has hardly been outside for over a year.
Comment:
Audrey’s daily supplements are clearly working well for her. She has achieved a level not only
of over 50 nmol/L but also over 75 nmol/L, which is a level that many suggest we should all be
aspiring to. She did better than Jen, the junior doctor below and one of her six grandchildren!
Turn round:
Considering the laboratory has had Christmas and New Year, not to mention special working
for social distancing, the turn-round of 4 working days is exceptionally good.
January 2021 No. 1
Secret Shopper: Jen
A 31 year old hospital doctor in London currently working extra shifts in the back of an
ambulance helping transfer Covid-19 patients between ITUs. She is vegetarian and spends a
lot of time outdoors. She does not take vitamin supplements.
Comment:
Jen was surprised that she was deficient. She intends taking several 25 ug (1,000 IU) vitamin
D3 tablets/day to get her level up. We have offered her a retest next month.
Turn round:
The service is hugely busy at present. Considering this sample arrived just two days before
Christmas, a turn-round of 10 working days is pretty good. We have set up a second secret
shopper in January to see how things are going.
December 2020
Secret Shopper: Chris
Chris was our second secret shopper from November. He sent his sample back the same time
as his wife, whose results are below. Unfortunately his spots were not very good and the
laboratory was not happy to analyse them and sent Chris a free of charge pack to try again.
Second time round his spots were just as bad - there was not enough blood to go right
through the filter paper. So…… the laboratory kindly sent Chris a third test pack, again free of
charge. This time Chris went the extra mile and there was enough blood on the card to
analyse!
Comments:
•
Chris has a reasonable level of Vitamin D at 56.1 nmol/L. However he does take two daily
supplements containing Vitamin D and he was a bit surprised it was not higher.
•
The laboratory does not analyse samples that might give misleading results, but rather sends
out a fresh pack if there are concerns about the blood spot appearance. This can be for
samples that are insufficient, and also where it looks like there has been ‘overspotting’, that is
several drops of blood placed on top of each other.
Turn round - the return of the sample by first class post from Chris was reasonable
considering it was the week before Christmas. The laboratory turned the result round in less
than 3 working days which is excellent considering how busy they are with their work tripling
over the last year due to concerns about getting Vitamin D levels right during the pandemic.
November 2020
Secret Shoppers: Judith & Chris
•
We have two secret shoppers this month. Judith and Christopher live together in Kent and
are retired. They both take cod liver oil every day and also a multi-vitamin tablet. They put
their samples in the post at the same time in separate pre-paid envelopes.
Comments:
•
Judith’s sample took over a week to arrive - the lab is experiencing some postal delays at
present. Once in the laboratory the sample was analysed in 4 working days and reported
back by email the same day. Considering the laboratory staff are all coping with the second
lock-down and a huge growth in workload, this is extremely impressive. Judith has a good
level of Vitamin D which would be expected from her long-term oral supplementation.
•
Chris’s results have not come back yet which is not unusual. Chris and Judith’s samples may
have been on separate runs, or the lab may have needed to reanalyse Chris’s sample as they
wanted to check the result.
October 2020
Secret shopper: Sue
•
Su is a 28 year old female engineer who has worked mainly from home in Birmingham since
lock-down. She takes a 25ug (1,000 IU) tablet of Vitamin D3 daily.
Comments:
•
Su has an adequate level but without her supplement she would almost certainly have been
in the ‘insufficiency’ category.
•
Turn-round is at the upper end of the laboratory target, at 9 working days. The laboratory
had some machine problems in mid-October requiring a service engineer for the LC-MS/MS
equipment.
September 2020
Secret shopper: Richard
•
Richard is a 61 year old male, who works outdoors as a lawn care expert in
Warwickshire, and has never taken vitamin D supplements.
Comments:
•
Laboratory: Turn round excellent at <2 working days in lab.
•
Result: Richard’s level is in the ADEQUATE category. He was slightly underwhelmed, as he thought his Vitamin D
would be higher with all his time outdoors over a sunny summer. He intends to take a supplement now through
the winter. We might use him again in March and see how his level kept up without the sun.